The Porcine IgG (Immunoglobulin G) ELISA Kit is a specialized assay designed for the precise and quantitative detection of IgG levels in porcine biological samples. Immunoglobulin G, a crucial component of the humoral immune response, plays a fundamental role in host defense mechanisms against pathogens and foreign antigens. As the most abundant antibody isotype in circulation, IgG mediates various immune functions, including pathogen neutralization, opsonization, and immune complex formation. This ELISA kit is designed to accurately quantify IgG concentrations in porcine samples, providing valuable insights into the humoral immune response in swine models. By measuring IgG levels, researchers can assess the antibody-mediated immune response, monitor immune status, and investigate the role of IgG in host defense and infection control in porcine studies. The Porcine IgG ELISA Kit offers exceptional sensitivity and specificity, enabling reliable and reproducible results. Manufactured under stringent quality control standards, this kit ensures robust performance and ease of use, making it an ideal tool for investigating immune responses, immunity, and infectious diseases in pigs.
Product Name:
Porcine IgG (Immunoglobulin G) ELISA Kit
SKU:
AEES00705
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
4 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
