The Porcine PDGFB (Platelet-Derived Growth Factor Subunit B) ELISA Kit from AssayGenie is a powerful tool for accurate and precise measurement of PDGFB levels in porcine samples. This kit is designed for use with serum, plasma, and cell culture supernatants, offering researchers a reliable and reproducible method for analyzing PDGFB levels in various experimental settings. PDGFB is a key growth factor involved in cell proliferation, migration, and angiogenesis. Dysregulation of PDGFB signaling has been implicated in a range of diseases, including cancer, fibrosis, and cardiovascular disorders, highlighting its importance as a potential therapeutic target.
By accurately quantifying PDGFB levels in porcine samples, researchers can gain valuable insights into the role of this growth factor in disease pathogenesis and progression. With its high sensitivity and specificity, the Porcine PDGFB ELISA Kit from AssayGenie is a valuable tool for understanding the biological functions of PDGFB and its potential as a biomarker for disease diagnosis and treatment. Trust AssayGenie for reliable ELISA kits that deliver precise and informative results for your research needs.
Product Name:
Porcine PDGFB ELISA Kit
SKU:
PRFI00132
Reactivity:
Porcine
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
28.125 pg/mL
Range:
46.875-3000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
91-101%
88-101%
86-99%
EDTA Plasma (n = 5)
82-100%
85-98%
85-100%
Heparin Plasma (n = 5)
85-100%
89-96%
85-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
91-104
94
EDTA Plasma (n = 5)
85-103
98
Heparin Plasma (n = 5)
86-105
98
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
