SynonymsCD62E,CD62-E,E-Selectin,ELAM,ELAM1,ESEL,SEL-E,E-LAM,E-LAM1,E-SEL,LECAM2,Endothelial Leukocyte Adhesion Molecule 1,CD62 Antigen-Like Family Member E
ReactivityPorcine
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
PRDL00137
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P98110
Sensitivity:
0.237ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
CD62E,CD62-E,E-Selectin,ELAM,ELAM1,ESEL,SEL-E,E-LAM,E-LAM1,E-SEL,LECAM2,Endothelial Leukocyte Adhesion Molecule 1,CD62 Antigen-Like Family Member E
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Porcine Selectin-Endothelium (Sele) ELISA Kit offered by Assay Genie is specifically designed for the quantitative detection of porcine Selectin-Endothelium levels in serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, researchers can rely on the accuracy and reproducibility of results obtained with this kit, making it suitable for a wide range of research applications.Selectin-Endothelium is a key adhesion molecule involved in the recruitment and binding of leukocytes to the endothelium during inflammation and immune response.
Monitoring Selectin-Endothelium levels can provide valuable insights into inflammatory processes, immune response, and vascular health in porcine models. With the Porcine Selectin-Endothelium (Sele) ELISA Kit from Assay Genie, researchers can confidently study the role of Selectin-Endothelium in various physiological and pathological conditions, aiding in the development of novel therapies and interventions in porcine models.
Product Name:
Porcine Selectin, Endothelium (SELE) ELISA Kit
Product Code:
PRDL00137
Size:
96 Assays
Target:
SELE
Synonyms:
CD62E, CD62-E, E-Selectin, ELAM, ELAM1, ESEL, SEL-E, E-LAM, E-LAM1, E-SEL, LECAM2, Endothelial Leukocyte Adhesion Molecule 1, CD62 Antigen-Like Family Member E
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
