Western blot analysis of extracts of various cell lines, using POU2F2 antibody (CAB21297) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The POU2F2 Polyclonal Antibody (CAB21297) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has been validated for use in various applications, including Western blotting and immunohistochemistry.POU2F2 is a transcription factor that regulates the expression of genes involved in immune response and cell differentiation. Its function in B cell development and activation makes it an important target for research in immunology and cancer biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
POU2F2 Polyclonal Antibody
SKU:
CAB21297
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OCT2, OTF2, Oct-2, POU2F2
Positive Sample:
Raji, Daudi, HepG2, NIH/3T3
Cellular Localization:
Cytoplasm.
Calculated MW:
51kDa
Observed MW:
60-75kDa
The protein encoded by this gene is a homeobox-containing transcription factor of the POU domain family. The encoded protein binds the octamer sequence 5'-ATTTGCAT-3', a common transcription factor binding site in immunoglobulin gene promoters. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5452
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using POU2F2 Rabbit pAb (CAB21297) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
