Anti-PPA1 Antibody (CAB2108)

Antibody Name:	Anti-PPA1 Antibody
Antibody SKU:	CAB2108
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PPA1 (NP_066952.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2, HT-29, Mouse liver, Mouse intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PPA1 (NP_066952.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSGF STEE RAAP FSLE YRVF LKNE KGQY ISPF HDIP IYAD KDVF HMVV EVPR WSNA KMEI ATKD PLNP IKQD VKKG KLRY VANL FPYK GYIW NYGA IPQT WEDP GHND KHTG CCGD NDPI DVCE IGSK VCAR GEII GVKV LGIL AMID EGET DWKV IAIN VDDP DAAN YNDI NDVK RLKP GYLE ATVD WFRR YKVP DGKP ENEF AFNA EFKD KDFA IDII KSTH DHWK ALVT KKTN GKGI SCMN TTLS ESPF KCDP DAAR AIVD ALPP PCES ACTV PTDV DKWF HHQK N
Gene ID:	5464
Uniprot:	Q15181
Cellular Location:	Cytoplasm
Calculated MW:	32kDa
Observed MW:	35kDa

Synonyms:	PPA1, HEL-S-66p, IOPPP, PP, PP1, SID6-8061
Background:	The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.

UniProt Protein Function:	PPA1: is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Hydrolase; EC 3.6.1.1; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 10q11.1-q24 Cellular Component: cytoplasm; cytosol Molecular Function:inorganic diphosphatase activity; magnesium ion binding Biological Process: tRNA aminoacylation for protein translation; gene expression; phosphate metabolic process
NCBI Summary:	The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15181
NCBI GenInfo Identifier:	8247940
NCBI Gene ID:	5464
NCBI Accession:	Q15181.2
UniProt Secondary Accession:	Q15181,Q2M348, Q5SQT7, Q6P7P4, Q9UQJ5, Q9Y5B1,
UniProt Related Accession:	Q15181
Molecular Weight:	289
NCBI Full Name:	Inorganic pyrophosphatase
NCBI Synonym Full Names:	pyrophosphatase (inorganic) 1
NCBI Official Symbol:	PPA1
NCBI Official Synonym Symbols:	PP; PP1; IOPPP; HEL-S-66p; SID6-8061
NCBI Protein Information:	inorganic pyrophosphatase; PPase; pyrophosphatase 1; inorganic diphosphatase; inorganic pyrophosphatase 1; diphosphate phosphohydrolase; pyrophosphate phospho-hydrolase; cytosolic inorganic pyrophosphatase; epididymis secretory sperm binding protein Li 66p
UniProt Protein Name:	Inorganic pyrophosphatase
UniProt Synonym Protein Names:	Pyrophosphate phospho-hydrolase; PPase
Protein Family:	Soluble inorganic pyrophosphatase
UniProt Gene Name:	PPA1
UniProt Entry Name:	IPYR_HUMAN

	Western blot analysis of extracts of various cell lines, using PPA1 antibody (CAB2108) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat brain using PPA1 Antibody (CAB2108) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using PPA1 Antibody (CAB2108) at dilution of 1:200 (40x lens).

Antibodies	ELISA Kits
Anti-PPA1 Antibody (CAB14050)	Human Inorganic Pyrophosphatase / PPA1 ELISA Kit
PPA1 Antibody (PACO11381)	Human Inorganic pyrophosphatase (PPA1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)