Anti-PPP1CB Antibody (CAB1088)

Antibody Name:	Anti-PPP1CB Antibody
Antibody SKU:	CAB1088
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human PPP1CB (NP_002700.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, HL-60, PC-3, HeLa, Mouse heart, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human PPP1CB (NP_002700.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADG ELNV DSLI TRLL EVRG CRPG KIVQ MTEA EVRG LCIK SREI FLSQ PILL ELEA PLKI CGDI HGQY TDLL RLFE YGGF PPEA NYLF LGDY VDRG KQSL ETIC LLLA YKIK YPEN FFLL RGNH ECAS INRI YGFY DECK RRFN IKLW KTFT DCFN CLPI AAIV DEKI FCCH GGLS PDLQ SMEQ IRRI MRPT DVPD TGLL CDLL WSDP DKDV QGWG ENDR GVSF TFGA DVVS KFLN RHDL DLIC RAHQ VVED GYEF FAKR QLVT LFSA PNYC GEFD NAGG MMSV DETL MCSF QILK PSEK KAKY QYGG LNSG RPVT PPRT ANPP KKR
Gene ID:	5500
Uniprot:	P62140
Cellular Location:	Cytoplasm, Nucleus, nucleolus, nucleoplasm
Calculated MW:	37kDa
Observed MW:	30-35kDa

Synonyms:

PPP1CB, HEL-S-80p, PP-1B, PP1B, PP1beta, PPP1CD, NSLH2

Background:

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.

UniProt Protein Function:	PPP1CB: is a catalytic subunit of protein phosphatase 1 (PP1), a ubiquitous cytosolic serine-threonine phosphatase. Composed a catalytic subunit (PPP1CA, PPP1CB or PPP1CC) which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting (or regulatory) subunits: PPP1R12A and PPP1R12B mediate binding to myosin; PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen; and PPP1R15A and PPP1R15B mediate binding to EIF2S1. PP1 is essential for cell division, plays critical roles in many cellular processes including glycogen metabolism, muscle contractility and protein synthesis, and is involved in the regulation of ionic conductances and long-term synaptic plasticity. Inhibits the synthesis of proteins involved in synaptic plasticity. Binds to the transcription factor cyclic AMP-dependent response element binding (CREB) and dephosphorylates it. May regulate chromatin condensation through regulation of histone H3 phosphorylation. Differentially expressed in gastric cancer. May participate in the neurodegenerative progress in Alzheimer's disease. Down-regulated in lung squamous cell carcinoma.
UniProt Protein Details:	Protein type:Nucleolus; Motility/polarity/chemotaxis; EC 3.1.3.16; EC 3.1.3.53; Protein phosphatase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 2p23 Cellular Component: nucleoplasm; focal adhesion; glycogen granule; nucleolus; protein phosphatase type 1 complex; nucleus Molecular Function:protein binding; myosin-light-chain-phosphatase activity; metal ion binding; phosphoric monoester hydrolase activity; myosin phosphatase activity; protein kinase binding Biological Process: regulation of cell adhesion; glycogen metabolic process; regulation of glycogen biosynthetic process; protein amino acid dephosphorylation; regulation of circadian rhythm; entrainment of circadian clock by photoperiod; regulation of glycogen catabolic process; cell division; transforming growth factor beta receptor signaling pathway; triacylglycerol catabolic process; circadian regulation of gene expression; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of transforming growth factor beta receptor signaling pathway
NCBI Summary:	The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:	P62140
NCBI GenInfo Identifier:	49065814
NCBI Gene ID:	5500
NCBI Accession:	P62140.3
UniProt Secondary Accession:	P62140,P37140, Q5U087, Q6FG45, B2R5V4, D6W565,
UniProt Related Accession:	P62140
Molecular Weight:	327
NCBI Full Name:	Serine/threonine-protein phosphatase PP1-beta catalytic subunit
NCBI Synonym Full Names:	protein phosphatase 1, catalytic subunit, beta isozyme
NCBI Official Symbol:	PPP1CB
NCBI Official Synonym Symbols:	PP1B; PP-1B; PPP1CD; PP1beta; HEL-S-80p
NCBI Protein Information:	serine/threonine-protein phosphatase PP1-beta catalytic subunit; protein phosphatase 1-beta; protein phosphatase 1-delta; epididymis secretory sperm binding protein Li 80p; protein phosphatase 1, catalytic subunit, beta isoform; protein phosphatase 1, cat
UniProt Protein Name:	Serine/threonine-protein phosphatase PP1-beta catalytic subunit
UniProt Gene Name:	PPP1CB
UniProt Entry Name:	PP1B_HUMAN

	Western blot analysis of extracts of various cell lines, using PPP1CB antibody (CAB1088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded Mouse lung using PPP1CB Rabbit pAb (CAB1088) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat lung using PPP1CB Rabbit pAb (CAB1088) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human liver using PPP1CB Rabbit pAb (CAB1088) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PPP1CB antibody (CAB1088). Blue: DAPI for nuclear staining.

Antibodies

Anti-PPP1CB Antibody (CAB13528)

Anti-PPP1CB Antibody (CAB13529)

Anti-Protein Phosphatase 1 beta Antibody (CAB4364)

PPP1CB Antibody (PACO11416)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)