Anti-PPP1R12A Antibody (CAB0587)

Antibody Name:	Anti-PPP1R12A Antibody
Antibody SKU:	CAB0587
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PPP1R12A (NP_002471.1).

Application:	WB IP
Recommended Dilution:	WB 1:500 - 1:2000 IP 1:50 - 1:100
Reactivity:	Human
Positive Samples:	U-87MG, LO2, Jurkat, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PPP1R12A (NP_002471.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKMA DAKQ KRNE QLKR WIGS ETDL EPPV VKRQ KTKV KFDD GAVF LAAC SSGD TDEV LKLL HRGA DINY ANVD GLTA LHQA CIDD NVDM VKFL VENG ANIN QPDN EGWI PLHA AASC GYLD IAEF LIGQ GAHV GAVN SEGD TPLD IAEE EAME ELLQ NEVN RQGV DIEA ARKE EERI MLRD ARQW LNSG HIND VRHA KSGG
Gene ID:	4659
Uniprot:	O14974
Cellular Location:	Cytoplasm
Calculated MW:	105kDa/109kDa/111kDa/115kDa
Observed MW:	140kDa

Synonyms:

PPP1R12A, M130, MBS, MYPT1, MYPT1

Background:

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit Chromosomal Location of Human Ortholog: 12q15-q21 Cellular Component: A band; actin cytoskeleton; centrosome; contractile fiber; cytoplasm; cytosol; focal adhesion; kinetochore; nucleoplasm; Z disc Molecular Function:enzyme inhibitor activity; phosphatase regulator activity; phosphoprotein phosphatase activity; protein binding; protein kinase binding; signal transducer activity Biological Process: centrosome organization and biogenesis; G2/M transition of mitotic cell cycle; mitosis; mitotic cell cycle; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; regulation of cell adhesion; regulation of nucleocytoplasmic transport; small GTPase mediated signal transduction
NCBI Summary:	Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]
UniProt Code:	O14974
NCBI GenInfo Identifier:	41017262
NCBI Gene ID:	4659
NCBI Accession:	O14974.1
UniProt Secondary Accession:	O14974,Q2NKL4, Q569H0, Q86WU3, Q8NFR6, Q9BYH0, B4DZ09 F8VWB4,
UniProt Related Accession:	O14974
Molecular Weight:	105,641 Da
NCBI Full Name:	Protein phosphatase 1 regulatory subunit 12A
NCBI Synonym Full Names:	protein phosphatase 1 regulatory subunit 12A
NCBI Official Symbol:	PPP1R12A
NCBI Official Synonym Symbols:	MBS; M130; MYPT1
NCBI Protein Information:	protein phosphatase 1 regulatory subunit 12A
UniProt Protein Name:	Protein phosphatase 1 regulatory subunit 12A
UniProt Synonym Protein Names:	Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit
Protein Family:	Protein phosphatase 1 regulatory
UniProt Gene Name:	PPP1R12A
UniProt Entry Name:	MYPT1_HUMAN

	Western blot analysis of extracts of various cell lines, using PPP1R12A antibody (CAB0587) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug PPP1R12A antibody (CAB0587). Western blot was performed from the immunoprecipitate using PPP1R12A antibody (CAB0587) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-PPP1R12A Antibody (CAB6700)	MYPT1 Colorimetric Cell-Based ELISA Kit
Anti-Phospho-PPP1R12A-S507 pAb Antibody (CABP0769)
Anti-Phospho-PPP1R12A-S668 pAb Antibody (CABP0835)
Anti-Phospho-PPP1R12A-T696 Antibody (CABP0915)
Anti-Phospho-PPP1R12A-T853 Antibody (CABP0916)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)