Description
pptT Recombinant Protein (N-His)
pptT Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Leu227, with an N-terminal His tag. Predicted molecular weight: 26.87 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. 4'-phosphopantetheinyl transferase PptT is a ~24 kDa protein. Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein. For research use only.
Involved in post-translational modification of various type-I polyketide synthases required for the formation of both mycolic acids and lipid virulence factors. Acts on Pks13, Mas, PpsA, PpsB, PpsC and PpsD. Also acts on AcpM, the meromycolate extension acyl carrier protein. In addition, is involved in the activation of the acyl carrier protein MbtL and the nonribosomal peptides synthases MbtB and MbtE, which are involved in the biosynthesis of the siderophore mycobactin.
| Product Name: | pptT Recombinant Protein (N-His) |
| SKU: | GXRP09965 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mycobacterium tuberculosis |
| Synonyms: | 4'-phosphopantetheinyl transferase PptT; PPTase; 2.7.8.7; pptT; Rv2794c |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Leu227 |
| Calculated MW: | 26.87 kDa |
| UniProt: | O33336 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
