Anti-PRDM2 Antibody (CAB8565)

Antibody Name:	Anti-PRDM2 Antibody
Antibody SKU:	CAB8565
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PRDM2 (NP_036363.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Rat
Positive Samples:	Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PRDM2 (NP_036363.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNQN TTEP VAAT ETLA EVPE HVLR GLPE EVRL FPSA VDKT RIGV WATK PILK GKKF GPFV GDKK KRSQ VKNN VYMW EVYY PNLG WMCI DATD PEKG NWLR YVNW ACSG EEQN LFPL EINR AIYY KTLK PIAP GEEL LVWY NGED NPEI AAAI EEER ASAR SKRS SPKS RKGK KKSQ ENKN KGNK IQDI QLKT SEPD FTSA NMRD SAEG PKED EEKP SASA LEQP ATLQ EVAS QEVP PELA
Gene ID:	7799
Uniprot:	Q13029
Cellular Location:	Nucleus
Calculated MW:	25kDa/162kDa/166kDa/184kDa/188kDa
Observed MW:	250kDa

Synonyms:

PRDM2, HUMHOXY1, KMT8, KMT8A, MTB-ZF, RIZ, RIZ1, RIZ2

Background:

This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	KSR: a serine/threonine kinase of the Raf family with no demonstrated kinase activity. A location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. Interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. In unstimulated cells, the phosphorylated form is bound to a 14-3-3 protein and sequestered in the cytoplasm. Translocates from the cytoplasm to the cell periphery following TNF treatment. When Bad is available, ceramide signaling through the Ras/Raf cascade can lead to apoptosis. Three alternatively-spliced isoforms have been described.
UniProt Protein Details:	Protein type:Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; RAF family Chromosomal Location of Human Ortholog: 17q11.2 Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum Molecular Function:protein serine/threonine kinase activity; MAP-kinase scaffold activity; protein binding; mitogen-activated protein kinase kinase binding; metal ion binding; ATP binding; protein kinase activity Biological Process: positive regulation of MAPKKK cascade; Ras protein signal transduction; protein amino acid phosphorylation
NCBI Summary:	This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
UniProt Code:	Q13029
NCBI GenInfo Identifier:	20336258
NCBI Gene ID:	7799
NCBI Accession:	NP_036363
UniProt Related Accession:	Q13029
Molecular Weight:
NCBI Full Name:	PR domain zinc finger protein 2 isoform a
NCBI Synonym Full Names:	PR/SET domain 2
NCBI Official Symbol:	PRDM2
NCBI Official Synonym Symbols:	RIZ; KMT8; RIZ1; RIZ2; KMT8A; MTB-ZF; HUMHOXY1
NCBI Protein Information:	PR domain zinc finger protein 2
UniProt Protein Name:	Kinase suppressor of Ras 1
Protein Family:	Kinase suppressor of Ras
UniProt Gene Name:	KSR1
UniProt Entry Name:	KSR1_HUMAN

	Western blot analysis of extracts of rat heart, using PRDM2 antibody (CAB8565) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat testis using PRDM2 antibody (CAB8565) at dilution of 1:100 (40x lens).

Antibodies

Anti-PRDM2 Antibody (CAB13157)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)