The PRMT8 Polyclonal Antibody (CAB2326) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high specificity and sensitivity for PRMT8 in human samples, making it suitable for use in Western blot applications. By binding to PRMT8, this antibody allows for the detection and analysis of PRMT8 levels in different cell types, making it ideal for studies in neurobiology and cancer research.PRMT8, a member of the protein arginine methyltransferase (PRMT) family, plays a crucial role in modulating gene expression through the post-translational modification of proteins.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PRMT8 Polyclonal Antibody
SKU:
CAB2326
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HRMT1L3, HRMT1L4, PRMT8
Positive Sample:
Jurkat, MCF7, SH-SY5Y
Cellular Localization:
Cell Membrane, Cytoplasmic Side, Lipid-Anchor.
Calculated MW:
45kDa
Observed MW:
45kDa
Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).
Purification Method
Affinity purification
Gene ID
56341
RRID
AB_2764291
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRMT8 Rabbit pAb (CAB2326). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of various lysates using PRMT8 Rabbit pAb (CAB2326) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
