Description
Protein A/SPA Recombinant Protein (C-His)
Protein A/SPA Recombinant Protein (C-His) is a recombinant protein expressed in E. coli, spanning Ala37-Lys327, with a C-terminal His tag. Predicted molecular weight: 33.88 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from PBS pH 7.4, 1 mM EDTA, 4% trehalose, 1% mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Immunoglobulin G-binding protein A (SPA) is a ~56 kDa protein. Plays a role in the inhibition of the host innate and adaptive immune responses. For research use only.
Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins. In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin.
| Product Name: | Protein A/SPA Recombinant Protein (C-His) |
| SKU: | GXRP03736 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Staphylococcus aureus |
| Synonyms: | Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA, spa, Protein A |
| Tag: | C-His |
| Expression Host: | E. coli |
| Protein Length: | Ala37-Lys327 |
| Calculated MW: | 33.88 kDa |
| UniProt: | P02976 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from PBS pH 7.4, 1 mM EDTA, 4% trehalose, 1% mannitol |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
