Anti-DR1 Antibody (CAB6903)

Antibody Name:	Anti-DR1 Antibody
Antibody SKU:	CAB6903
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-176 of human DR1 (NP_001929.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, THP-1, Jurkat, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-176 of human DR1 (NP_001929.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASS SGND DDLT IPRA AINK MIKE TLPN VRVA NDAR ELVV NCCT EFIH LISS EANE ICNK SEKK TISP EHVI QALE SLGF GSYI SEVK EVLQ ECKT VALK RRKA SSRL ENLG IPEE ELLR QQQE LFAK ARQQ QAEL AQQE WLQM QQAA QQAQ LAAA SASA SNQA GSSQ DEED DDDI
Gene ID:	1810
Uniprot:	Q01658
Cellular Location:	Nucleus
Calculated MW:	19kDa
Observed MW:	19kDa

Synonyms:

DR1, NC2, NC2-BETA, NC2B, NCB2

Background:

This gene encodes a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription.

UniProt Protein Function:	DR1: The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Heterodimer with DRAP1. DR1 exists in solution as a homotetramer that dissociates during interaction with TBP and then, after complexing with TBP, reassociates at a slow rate, to reconstitute the tetramer. Interacts with NFIL3. Component of the ADA2A-containing complex (ATAC), composed of CSRP2BP, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. Belongs to the NC2 beta/DR1 family.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 1p22.1 Cellular Component: nucleus Molecular Function:protein binding; TATA-binding protein binding; transcription corepressor activity; transcription factor binding Biological Process: negative regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription. [provided by RefSeq, Jul 2008]
UniProt Code:	Q01658
NCBI GenInfo Identifier:	401162
NCBI Gene ID:	1810
NCBI Accession:	Q01658.1
UniProt Related Accession:	Q01658
Molecular Weight:	19,444 Da
NCBI Full Name:	Protein Dr1
NCBI Synonym Full Names:	down-regulator of transcription 1
NCBI Official Symbol:	DR1
NCBI Official Synonym Symbols:	NC2; NC2B; NC2-BETA
NCBI Protein Information:	protein Dr1
UniProt Protein Name:	Protein Dr1
UniProt Synonym Protein Names:	Down-regulator of transcription 1; Negative cofactor 2-beta; NC2-beta; TATA-binding protein-associated phosphoprotein
Protein Family:	Dr1-associated corepressor
UniProt Gene Name:	DR1
UniProt Entry Name:	NC2B_HUMAN

	Western blot analysis of extracts of various cell lines, using DR1 antibody (CAB6903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat kidney using DR1 Antibody (CAB6903) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using DR1 Antibody (CAB6903) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using DR1 antibody (CAB6903).

Antibodies

Anti-DR1 Antibody (CAB13298)

DR1 Antibody (PACO08933)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)