The PRPF4B Antibody (CAB14815) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for detection and analysis in Western blot applications. By targeting PRPF4B, researchers can explore the intricate mechanisms of RNA processing and gene expression regulation, providing valuable insights into various cellular processes and diseases related to splicing defects.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PRPF4B Antibody
SKU:
CAB14815
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PR4H, PRP4, PRP4H, PRP4K, dJ1013A10.1, PRPF4B
Positive Sample:
293T, A-549, HeLa, Mouse liver
Cellular Localization:
Nucleus.
Calculated MW:
117kDa
Observed MW:
160kDa
Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs).
Purification Method
Affinity purification
Gene ID
8899
RRID
AB_2761693
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRPF4B Rabbit pAb (CAB14815) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
