PSD-95 Antibody [B14] is a rabbit recombinant monoclonal antibody directed against PSD-95, offered by Assay Genie for research applications. Reported applications include WB, IHC and ICC/IF. Reported reactivity: Human, Mouse and Rat. Supplied as IgG Kappa, purified by AmMag™ Ultra AT Protein A MagBeads.
PSD95 (Postsynaptic Density Protein 95), also known as SAP90, is a scaffolding protein in the membrane-associated guanylate kinase (MAGUK) family. It organizes the postsynaptic density by anchoring receptors, ion channels, and signaling molecules at excitatory synapses. PSD95 contains three PDZ domains, an SH3 domain, and a guanylate kinase-like domain. The PDZ domains mediate interactions with NMDA receptors, potassium channels, and neuroligins, facilitating synaptic signaling and plasticity. PSD95 also binds neuronal nitric oxide synthase, linking it to redox signaling. Through its role in AMPA receptor trafficking via Stargazin and TARPs, PSD95 influences synaptic strength and experience-dependent plasticity. Mutations in PSD95 are associated with autism spectrum disorders and cognitive deficits, underscoring its importance in learning and memory and its relevance in neurodegenerative disease research. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
![Western blot analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228) showing detection of Human PSD95 protein in a transient over-expression (OE) lysate (OriGene LY419977). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:10,000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/279994/759169/anti-psd-95-antibody-b14__17328.1785505165.jpg?c=2?imbypass=on "Western blot analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228) showing detection of Human PSD95 protein in a transient over-expression (OE) lysate (OriGene LY419977). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:10,000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:100 for 1 hour at RT.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/279994/774391/anti-psd-95-antibody-b14__66716.1785553297.jpg?c=2?imbypass=on "Immunocytochemistry/Immunofluorescence analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:100 for 1 hour at RT.")
![Immunohistochemistry analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228). Tissue: Brain. Species: Mouse. Fixation: Paraffin embedded, 4um thin. Blocking: 3% milk for 1 hour at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:100 for 45 min at RT.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/279994/762183/anti-psd-95-antibody-b14__71835.1785514662.jpg?c=2?imbypass=on "Immunohistochemistry analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228). Tissue: Brain. Species: Mouse. Fixation: Paraffin embedded, 4um thin. Blocking: 3% milk for 1 hour at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:100 for 45 min at RT.")
![Immunocytochemistry/Immunofluorescence analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228). Tissue: Neuroblastoma cells (Kelly). Species: Human. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:100 for 1 hour at RT.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/279994/763000/anti-psd-95-antibody-b14__83263.1785517283.jpg?c=2?imbypass=on "Immunocytochemistry/Immunofluorescence analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228). Tissue: Neuroblastoma cells (Kelly). Species: Human. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:100 for 1 hour at RT.")
![Western blot analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228) showing detection of Rat PSD95 protein in a Rat brain membrane lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/279994/757734/SMC-624_PSD95_Antibody_B14_WB_Rat_Brain-membrane-Lysate_1__23328.1785491718.png?c=2?imbypass=on "Western blot analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228) showing detection of Rat PSD95 protein in a Rat brain membrane lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.")
![Western blot analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228) showing detection of Human PSD95 protein in a transient over-expression (OE) lysate (OriGene LY419977). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:10,000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/279994/759169/anti-psd-95-antibody-b14__17328.1785505165.jpg?c=2 "Western blot analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228) showing detection of Human PSD95 protein in a transient over-expression (OE) lysate (OriGene LY419977). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:10,000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.")
