Anti-PSMA7 Antibody (CAB4052)

Antibody Name:	Anti-PSMA7 Antibody
Antibody SKU:	CAB4052
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human PSMA7 (NP_002783.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, 293T, MCF7, Mouse brain, Mouse liver, Mouse lung, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human PSMA7 (NP_002783.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSYD RAIT VFSP DGHL FQVE YAQE AVKK GSTA VGVR GRDI VVLG VEKK SVAK LQDE RTVR KICA LDDN VCMA FAGL TADA RIVI NRAR VECQ SHRL TVED PVTV EYIT RYIA SLKQ RYTQ SNGR RPFG ISAL IVGF DFDG TPRL YQTD PSGT YHAW KANA IGRG AKSV REFL EKNY TDEA IETD DLTI KLVI KALL EVVQ SGGK NIEL AVMR RDQS LKIL NPEE IEKY VAEI EKEK EENE KKKQ KKAS
Gene ID:	5688
Uniprot:	O14818
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	16kDa/20kDa/27kDa
Observed MW:	30kDa

Synonyms:

PSMA7, C6, HEL-S-276, HSPC, RC6-1, XAPC7

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the peptidase T1A family that functions as a 20S core alpha subunit. The encoded protein interacts with the hepatitis B virus X protein and plays a role in regulating hepatitis C virus internal ribosome entry site (IRES) activity, an activity essential for viral replication. The encoded protein also plays a role in the cellular stress response by regulating hypoxia-inducible factor-1alpha. A pseudogene of this gene is located on the long arm of chromosome 9.

UniProt Protein Function:	PSMA7: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis. Interacts with some important proteins involved in transcription factor regulation, cell cycle transition, viral replication and even tumor initiation and progression. Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA7 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Interacts with HIV-1 TAT protein. Interacts with hepatitis B virus X protein (HBX). Interacts with HIF1A. Interacts with RAB7A. Interacts with PARK2. Interacts with ABL1 and ABL2. Interacts with EMAP2. Interacts with MAVS. Down-regulated by the ribozyme Rz3'X. Up-regulated in colorectal cancer tissues. Belongs to the peptidase T1A family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Proteasome complex; EC 3.4.25.1; Protease Chromosomal Location of Human Ortholog: 20q13.33 Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; nucleus; cytosol Molecular Function:threonine endopeptidase activity; identical protein binding; protein binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the peptidase T1A family that functions as a 20S core alpha subunit. The encoded protein interacts with the hepatitis B virus X protein and plays a role in regulating hepatitis C virus internal ribosome entry site (IRES) activity, an activity essential for viral replication. The encoded protein also plays a role in the cellular stress response by regulating hypoxia-inducible factor-1alpha. A pseudogene of this gene is located on the long arm of chromosome 9. [provided by RefSeq, Jul 2012]
UniProt Code:	O14818
NCBI GenInfo Identifier:	12643540
NCBI Gene ID:	5688
NCBI Accession:	O14818.1
UniProt Secondary Accession:	O14818,Q5JXJ2, Q9BR53, Q9H4K5, Q9UEU8, B2R515,
UniProt Related Accession:	O14818
Molecular Weight:	248
NCBI Full Name:	Proteasome subunit alpha type-7
NCBI Synonym Full Names:	proteasome (prosome, macropain) subunit, alpha type, 7
NCBI Official Symbol:	PSMA7
NCBI Official Synonym Symbols:	C6; HSPC; RC6-1; XAPC7
NCBI Protein Information:	proteasome subunit alpha type-7; proteasome subunit RC6-1; proteasome subunit XAPC7; proteasome subunit alpha 4
UniProt Protein Name:	Proteasome subunit alpha type-7
UniProt Synonym Protein Names:	Proteasome subunit RC6-1; Proteasome subunit XAPC7
Protein Family:	Proteasome
UniProt Gene Name:	PSMA7
UniProt Entry Name:	PSA7_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMA7 antibody (CAB4052) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts of rat liver, using PSMA7 antibody (CAB4052) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat testis using PSMA7 antibody (CAB4052) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using PSMA7 antibody (CAB4052) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using PSMA7 antibody (CAB4052) at dilution of 1:100 (40x lens).