Anti-PSMB2 Antibody (CAB5483)

Antibody Name:	Anti-PSMB2 Antibody
Antibody SKU:	CAB5483
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human PSMB2 (NP_002785.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T, SH-SY5Y, Mouse brain, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human PSMB2 (NP_002785.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEYL IGIQ GPDY VLVA SDRV AASN IVQM KDDH DKMF KMSE KILL LCVG EAGD TVQF AEYI QKNV QLYK MRNG YELS PTAA ANFT RRNL ADCL RSRT PYHV NLLL AGYD EHEG PALY YMDY LAAL AKAP FAAH GYGA FLTL SILD RYYT PTIS RERA VELL RKCL EELQ KRFI LNLP TFSV RIID KNGI HDLD NISF PKQG S
Gene ID:	5690
Uniprot:	P49721
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	22kDa
Observed MW:	23KDa

Synonyms:

PSMB2, HC7-I

Background:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt Protein Function:	PSMB2: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity. Belongs to the peptidase T1B family.
UniProt Protein Details:	Protein type:EC 3.4.25.1; Cell cycle regulation; Proteasome complex; Protease Chromosomal Location of Human Ortholog: 1p34.2 Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; membrane; cytosol; nucleus Molecular Function:threonine endopeptidase activity; protein binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; response to organic nitrogen; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to organic cyclic substance; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
NCBI Summary:	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
UniProt Code:	P49721
NCBI GenInfo Identifier:	1709762
NCBI Gene ID:	5690
NCBI Accession:	P49721.1
UniProt Secondary Accession:	P49721,P31145, Q9BWZ9, D3DPS0,
UniProt Related Accession:	P49721
Molecular Weight:	201
NCBI Full Name:	Proteasome subunit beta type-2
NCBI Synonym Full Names:	proteasome (prosome, macropain) subunit, beta type, 2
NCBI Official Symbol:	PSMB2
NCBI Official Synonym Symbols:	HC7-I
NCBI Protein Information:	proteasome subunit beta type-2; macropain subunit C7-I; proteasome beta 2 subunit; proteasome component C7-I; proteasome subunit, beta type, 2; multicatalytic endopeptidase complex subunit C7-1; multicatalytic endopeptidase complex subunit C7-I
UniProt Protein Name:	Proteasome subunit beta type-2
UniProt Synonym Protein Names:	Macropain subunit C7-I; Multicatalytic endopeptidase complex subunit C7-I; Proteasome component C7-I
Protein Family:	Proteasome
UniProt Gene Name:	PSMB2
UniProt Entry Name:	PSB2_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMB2 antibody (CAB5483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using PSMB2 antibody (CAB5483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human esophageal cancer using PSMB2 Rabbit pAb (CAB5483) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PSMB2 antibody (CAB5483). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug PSMB2 antibody (CAB5483). Western blot was performed from the immunoprecipitate using PSMB2 antibody (CAB5483) at a dilition of 1:1000.

Antibodies

Anti-PSMB2 Antibody (CAB13630)

PSMB2 Antibody (PACO11532)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)