Anti-PSME1 Antibody (CAB5358)

Antibody Name:	Anti-PSME1 Antibody
Antibody SKU:	CAB5358
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human PSME1 (NP_006254.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, 22Rv1, BT-474, Mouse spleen, Mouse liver, Mouse lung, Mouse heart, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human PSME1 (NP_006254.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAML RVQP EAQA KVDV FRED LCTK TENL LGSY FPKK ISEL DAFL KEPA LNEA NLSN LKAP LDIP VPDP VKEK EKEE RKKQ QEKE DKDE KKKG EDED KGPP CGPV NCNE KIVV LLQR LKPE IKDV IEQL NLVT TWLQ LQIP RIED GNNF GVAV QEKV FELM TSLH TKLE GFHT QISK YFSE RGDA VTKA AKQP HVGD YRQL VHEL DEAE YRDI RLMV MEIR NAYA VLYD IILK NFEK LKKP RGET KGMI Y
Gene ID:	5720
Uniprot:	Q06323
Cellular Location:
Calculated MW:	26kDa/28kDa
Observed MW:	31kDa

Synonyms:

PSME1, HEL-S-129m, IFI5111, PA28A, PA28alpha, REGalpha

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	PSME1: Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Heterodimer of PSME1 and PSME2, which forms a hexadimeric ring. PSME1 can form homoheptamers. By IFNG/IFN-gamma. Belongs to the PA28 family.
UniProt Protein Details:	Protein type:Proteasome complex Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: nucleoplasm; proteasome complex; proteasome activator complex; cytoplasm; cytosol Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:	Q06323
NCBI GenInfo Identifier:	1170519
NCBI Gene ID:	5720
NCBI Accession:	Q06323.1
UniProt Secondary Accession:	Q06323,Q6IBM2, Q9UEF4, A6NJG9, H0YNE3,
UniProt Related Accession:	Q06323
Molecular Weight:	249
NCBI Full Name:	Proteasome activator complex subunit 1
NCBI Synonym Full Names:	proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
NCBI Official Symbol:	PSME1
NCBI Official Synonym Symbols:	PA28A; IFI5111; REGalpha; PA28alpha
NCBI Protein Information:	proteasome activator complex subunit 1; IGUP I-5111; 29-kD MCP activator subunit; interferon-gamma IEF SSP 5111; 11S regulator complex subunit alpha; interferon-gamma-inducible protein 5111; interferon gamma up-regulated I-5111 protein; activator of multicatalytic protease subunit 1
UniProt Protein Name:	Proteasome activator complex subunit 1
UniProt Synonym Protein Names:	11S regulator complex subunit alpha; REG-alpha; Activator of multicatalytic protease subunit 1; Interferon gamma up-regulated I-5111 protein; IGUP I-5111; Proteasome activator 28 subunit alpha; PA28a; PA28alpha
Protein Family:	Proteasome activator complex
UniProt Gene Name:	PSME1
UniProt Entry Name:	PSME1_HUMAN

	Western blot analysis of extracts of various cell lines, using PSME1 antibody (CAB5358) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse heart using PSME1 antibody (CAB5358) at dilution of 1:100 (40x lens).

Antibodies

Anti-PSME1 Antibody (CAB5070)

PSME1 Antibody (PACO11556)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)