Anti-PTBP1 Antibody (CAB1831)

Antibody Name:	Anti-PTBP1 Antibody
Antibody SKU:	CAB1831
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PTBP1 (NP_114368.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, U-937, SW620

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PTBP1 (NP_114368.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDGI VPDI AVGT KRGS DELF STCV TNGP FIMS SNSA SAAN GNDS KKFK GDSR SAGV PSRV IHIR KLPI DVTE GEVI SLGL PFGK VTNL LMLK GKNQ AFIE MNTE EAAN TMVN YYTS VTPV LRGQ PIYI QFSN HKEL KTDS SPNQ ARAQ AALQ AVNS VQSG NLAL AASA AAVD AGMA MAGQ SPVL RIIV ENLF YPVT LDVL HQIF SKFG TVLK IITF TKNN QFQA LLQY ADPV SAQH AKLS LDGQ NIYN ACCT LRID FSKL TSLN VKYN NDKS RDYT RPDL PSGD SQPS LD
Gene ID:	5725
Uniprot:	P26599
Cellular Location:	Nucleus
Calculated MW:	57kDa/59kDa
Observed MW:	64kDa

Synonyms:

PTBP1, HNRNP-I, HNRNPI, HNRPI, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB

Background:

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.

UniProt Protein Function:	hnRNP I: a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP). hnRNPs are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. Binds to the polypyrimidine tract of introns. May promote the binding of U2 snRNP to pre-mRNA. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:RNA-binding; RNA splicing Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleoplasm; membrane; nucleolus Molecular Function:protein binding; poly-pyrimidine tract binding; RNA binding; nucleotide binding Biological Process: nuclear mRNA splicing, via spliceosome; negative regulation of RNA splicing; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; negative regulation of muscle cell differentiation; gene expression; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome
NCBI Summary:	This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P26599
NCBI GenInfo Identifier:	131528
NCBI Gene ID:	5725
NCBI Accession:	P26599.1
UniProt Secondary Accession:	P26599,Q9BUQ0,
UniProt Related Accession:	P26599
Molecular Weight:	531
NCBI Full Name:	Polypyrimidine tract-binding protein 1
NCBI Synonym Full Names:	polypyrimidine tract binding protein 1
NCBI Official Symbol:	PTBP1
NCBI Official Synonym Symbols:	PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I
NCBI Protein Information:	polypyrimidine tract-binding protein 1; hnRNP I; RNA-binding protein; 57 kDa RNA-binding protein PPTB-1; heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; polypyrimidine tract binding protein (heterogeneous
UniProt Protein Name:	Polypyrimidine tract-binding protein 1
UniProt Synonym Protein Names:	57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; hnRNP I
Protein Family:	Polypyrimidine tract-binding protein
UniProt Gene Name:	PTBP1
UniProt Entry Name:	PTBP1_HUMAN

	Western blot analysis of extracts of various cell lines, using PTBP1 antibody (CAB1831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat spleen using PTBP1 antibody (CAB1831) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using PTBP1 antibody (CAB1831) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using PTBP1 antibody (CAB1831) at dilution of 1:100 (40x lens).
	Confocal immunofluorescence analysis of U-2OS cells using PTBP1 antibody (CAB1831) at dilution of 1:50. Blue: DAPI for nuclear staining.

Antibodies

Anti-PTBP1 Antibody (CAB6107)

Anti-PTBP1 Antibody (CAB18052)[KO Validated]

Anti-PTBP1 Antibody (CAB18084)[KO Validated]

Anti-PTBP1 Antibody (CAB3487)

PTBP1 Antibody (PACO11565)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)