The Parvalbumin (PVALB) Antibody (CAB13538) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting PVALB in human samples, making it ideal for use in Western blot applications. By targeting the PVALB protein, researchers can gain insights into its function in muscle physiology and potentially uncover new therapeutic targets for muscle-related disorders.PVALB, also known as parvalbumin, is a calcium-binding protein found in fast-twitch muscle fibers and certain neurons. Its involvement in regulating intracellular calcium levels positions it as a key player in muscle contraction and relaxation mechanisms.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Parvalbumin (PVALB) Antibody
SKU:
CAB13538
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
D22S749, Parvalbumin (PVALB)
Positive Sample:
Mouse brain, Mouse skeletal muscle, Rat brain, Rat skeletal muscle
Cellular Localization:
Axon, Cytoplasm, Nucleus, Synapse.
Calculated MW:
12kDa
Observed MW:
15kDa
The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
5816
RRID
AB_2760398
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Parvalbumin (PVALB) Rabbit pAb (CAB13538) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of various lysates using Parvalbumin (PVALB) Rabbit pAb (CAB13538) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of paraffin-embedded Rat cerebellum tissue using Parvalbumin (PVALB) Rabbit pAb(CAB13538) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
