Anti-PDXK Antibody (CAB6687)

Antibody Name:	Anti-PDXK Antibody
Antibody SKU:	CAB6687
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human PDXK (NP_003672.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HT-29, HeLa, HepG2, Mouse liver, Mouse kidney, Mouse brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human PDXK (NP_003672.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEEE CRVL SIQS HVIR GYVG NRAA TFPL QVLG FEID AVNS VQFS NHTG YAHW KGQV LNSD ELQE LYEG LRLN NMNK YDYV LTGY TRDK SFLA MVVD IVQE LKQQ NPRL VYVC DPVL GDKW DGEG SMYV PEDL LPVY KEKV VPLA DIIT PNQF EAEL LSGR KIHS QEEA LRVM DMLH SMGP DTVV ITSS DLPS PQGS NYLI VLGS QRRR NPAG SVVM ERIR MDIR KVDA VFVG TGDL FAAM LLAW THKH PNNL KVAC EKTV STLH HVLQ RTIQ CAKA QAGE GVRP SPMQ LELR MVQS KRDI EDPE IVVQ ATVL
Gene ID:	8566
Uniprot:	O00764
Cellular Location:	Cytoplasm
Calculated MW:	26kDa/31kDa/35kDa
Observed MW:	35kDa

Synonyms:	PDXK, C21orf124, C21orf97, HEL-S-1a, PKH, PNK, PRED79
Background:	The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

UniProt Protein Function:	PDXK: Required for synthesis of pyridoxal-5-phosphate from vitamin B6. Belongs to the pyridoxine kinase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Kinase, other; Cofactor and Vitamin Metabolism - vitamin B6; EC 2.7.1.35 Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:ATP binding; lithium ion binding; magnesium ion binding; potassium ion binding; protein homodimerization activity; pyridoxal kinase activity; pyridoxal phosphate binding; sodium ion binding; zinc ion binding Biological Process: cell proliferation; pyridoxal 5'-phosphate salvage; pyridoxal phosphate biosynthetic process; vitamin B6 metabolic process
NCBI Summary:	The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	O00764
NCBI GenInfo Identifier:	2811007
NCBI Gene ID:	8566
NCBI Accession:	O00764.1
UniProt Secondary Accession:	O00764,Q7Z2Y0, Q9BS02,
UniProt Related Accession:	O00764
Molecular Weight:	26,827 Da
NCBI Full Name:	Pyridoxal kinase
NCBI Synonym Full Names:	pyridoxal (pyridoxine, vitamin B6) kinase
NCBI Official Symbol:	PDXK
NCBI Official Synonym Symbols:	PKH; PNK; PRED79; C21orf97; HEL-S-1a; C21orf124
NCBI Protein Information:	pyridoxal kinase
UniProt Protein Name:	Pyridoxal kinase
UniProt Synonym Protein Names:	Pyridoxine kinase
Protein Family:	Pyridoxine kinase
UniProt Gene Name:	PDXK
UniProt Entry Name:	PDXK_HUMAN

	Western blot analysis of extracts of various cell lines, using PDXK antibody (CAB6687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of U2OS cells using PDXK antibody (CAB6687). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
PDXK Antibody (PACO11155)	Human Pyridoxal Kinase / PDXK ELISA Kit
	Human PDXK (Pyridoxal Kinase) ELISA Kit (HUES02168)
	Human Pyridoxal kinase (PDXK) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)