The QPCT Antibody (CAB6711) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to QPCT, this antibody allows for the detection and analysis of QPCT protein levels in a variety of cell types, making it ideal for studies in endocrinology and peptide hormone regulation.QPCT, also known as glutaminyl-peptide cyclotransferase, plays a crucial role in the processing and regulation of peptide hormones, including the conversion of glutaminyl peptides into pyroglutamyl peptides.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
QPCT Antibody
SKU:
CAB6711
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
QC, GCT, sQC, QPCT
Positive Sample:
Mouse liver, Rat liver
Cellular Localization:
Secreted.
Calculated MW:
41kDa
Observed MW:
41kDa
This gene encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase.
Purification Method
Affinity purification
Gene ID
25797
RRID
AB_2767295
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using QPCT Rabbit pAb (CAB6711) at 1:2500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of MCF-7 cells using QPCT Rabbit pAb (CAB6711). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
