The QuickStep Human Cortisol ELISA Kit is specifically designed for the precise measurement of cortisol levels in human samples including serum, plasma, and saliva. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Cortisol, often referred to as the "stress hormone," plays a crucial role in the body's response to stress, metabolism, immune function, and more.
Monitoring cortisol levels can provide valuable insights into conditions such as stress-related disorders, adrenal dysfunction, and hormonal imbalances.With its ease of use and reliability, the QuickStep Human Cortisol ELISA Kit is an essential tool for researchers and clinicians studying the impact of cortisol on human health and developing targeted interventions for related conditions.
Product Name:
QuickStep Human Cortisol ELISA Kit
Product Code:
QSES020
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Human
Sensitivity:
2.87 ng/mL
Range:
6.25-400 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum, plasma, urine and saliva
Target:
Cortisol
Application:
This ELISA kit applies to the in vitro quantitative determination of Cortisol concentrations in serum, plasma, urine, saliva.
Specificity:
This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Human serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
18.87
58.54
147.36
18.78
63.49
141.75
Standard deviation
1.28
3.40
5.56
0.97
3.58
5.07
CV (%)
6.79
5.80
3.77
5.19
5.64
3.58
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
84-96
90
EDTA plasma(n=8)
90-103
96
Urine(n=8)
99
Samples were spiked with high concentrations of Human Cortisol and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
