The QuickStep Human IgA (Immunoglobulin A) ELISA Kit is a highly reliable and sensitive tool for the precise measurement of IgA levels in human biological samples such as serum, plasma, and cell culture supernatants. This advanced kit offers exceptional accuracy and reproducibility, making it an ideal choice for a variety of research applications.IgA is a crucial antibody that plays a key role in the body's immune response, particularly in mucosal immunity. Abnormal levels of IgA have been associated with a wide range of autoimmune diseases, allergies, and infections, making it a valuable biomarker for studying these conditions and developing potential treatments.With its high sensitivity and specificity, the QuickStep Human IgA ELISA Kit provides researchers with a powerful tool for investigating the role of IgA in various diseases and developing new therapeutic strategies.
Product Name:
Human IgA (Immunoglobulin A) Quickstep ELISA Kit
SKU:
QSES023
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
0.14 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
