The QuickStep Human T Testosterone ELISA Kit is a cutting-edge tool designed for the precise measurement of testosterone levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research applications.Testosterone is a vital hormone that plays a crucial role in regulating various physiological processes, including muscle growth, bone density, and reproductive function. Imbalances in testosterone levels have been linked to numerous health conditions, such as infertility, erectile dysfunction, and metabolic disorders.
By accurately measuring testosterone levels, researchers can gain valuable insights into these conditions and explore potential therapeutic interventions.Whether you are studying endocrine disorders, reproductive health, or sports performance, the QuickStep Human T Testosterone ELISA Kit offers a reliable and efficient solution for detecting and quantifying testosterone levels with precision. Trust in this innovative kit to streamline your research and unlock new possibilities in the field of hormone analysis.
Product Name:
QuickStep Human Testosterone ELISA Kit
Product Code:
QSES021
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Human
Sensitivity:
0.24 ng/mL
Range:
0.63-40 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum, plasma, urine and saliva
Target:
Testosterone
Application:
This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum, plasma, urine, saliva.
Specificity:
This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Human serum, plasma, saliva and urine samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human T are added to the micro ELISA plate wells. Human T in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human T. The concentration of Human T in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
2.04
4.88
18.80
2.01
4.96
18.17
Standard deviation
0.14
0.24
0.80
0.12
0.25
0.80
CV (%)
6.63
4.93
4.27
6.08
5.07
4.38
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
86-99
91
EDTA plasma(n=8)
94-106
100
Urine(n=8)
99
Samples were spiked with high concentrations of Human T and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
