The QuickStep Monkey E3 Estriol ELISA Kit is a cutting-edge solution for the precise measurement of E3 estriol levels in monkey serum, plasma, and cell culture samples. With its superior sensitivity and specificity, this kit delivers dependable and consistent results, making it a valuable tool for various research studies.E3 estriol is a key hormone that plays a vital role in reproductive health and pregnancy in monkeys.
Monitoring E3 estriol levels can provide valuable insights into hormonal balance, reproductive status, and overall health in these animals. This kit is essential for researchers studying reproductive biology, endocrinology, and hormone regulation in monkeys, offering a reliable method for assessing E3 estriol levels with accuracy and efficiency.
Product Name:
QuickStep Monkey Estriol ELISA Kit
Product Code:
QSES041
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Monkey
Sensitivity:
0.39 pg/mL
Range:
1.56-100 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Estriol
Application:
This ELISA kit applies to the in vitro quantitative determination of E3 concentrations in serum and plasma.
Specificity:
This kit recognizes E3 in samples. No significant cross-reactivity or interference between E3 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Monkey serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Monkey E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Monkey E3 are added to the micro ELISA plate wells. Monkey E3 in samples (or standards) competes with a fixed amount of Monkey E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to Monkey E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Monkey E3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
4.85
10.82
41.26
4.99
10.09
41.34
Standard deviation
0.29
0.51
1.67
0.26
0.59
2.10
CV (%)
6.00
4.69
4.04
5.18
5.89
5.09
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
89-104
96
EDTA plasma(n=8)
92-106
98
Samples were spiked with high concentrations of Monkey E3 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
