The QuickStep Porcine E3 Estriol ELISA Kit is a reliable and accurate tool for measuring E3 Estriol levels in porcine serum, plasma, and other biological samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for researchers working in the fields of reproductive health and endocrinology.E3 Estriol is a key hormone involved in the regulation of female reproductive function, particularly during pregnancy. Abnormal levels of E3 Estriol can indicate potential complications such as preeclampsia or fetal growth restriction, making it a valuable biomarker for monitoring pregnancy health and assessing fetal well-being.
Whether studying reproductive physiology, assessing fertility, or monitoring pregnancy, the QuickStep Porcine E3 Estriol ELISA Kit provides researchers with a powerful tool for accurate hormone analysis and insights into porcine reproductive health.
Product Name:
QuickStep Porcine Estriol ELISA Kit
Product Code:
QSES046
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Porcine
Sensitivity:
1.12 pg/mL
Range:
3.13-200 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Estriol
Application:
This ELISA kit applies to the in vitro quantitative determination of E3 concentrations in serum and plasma.
Specificity:
This kit recognizes E3 in samples. No significant cross-reactivity or interference between E3 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Porcine serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Porcine E3 are added to the micro ELISA plate wells. Porcine E3 in samples (or standards) competes with a fixed amount of Porcine E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to Porcine E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Porcine E3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
10.34
30.45
79.41
9.75
27.45
81.51
Standard deviation
0.65
1.75
2.80
0.49
1.27
3.86
CV (%)
6.30
5.76
3.53
5.04
4.64
4.74
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
89-103
96
EDTA plasma(n=8)
88-102
96
Samples were spiked with high concentrations of Porcine E3 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
