The QuickStep Rat E2 (Estradiol) ELISA Kit is specifically designed for the precise quantification of estradiol levels in rat serum, plasma, and tissue homogenates. This ELISA kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research applications.Estradiol, a key estrogen hormone, plays a critical role in regulating reproductive functions, bone health, and cardiovascular health in rats.
Monitoring estradiol levels is essential for studying hormonal imbalances, reproductive disorders, and potential treatments in rat models. The QuickStep Rat E2 ELISA Kit provides researchers with a reliable tool to assess estradiol levels and advance their research in the field of endocrinology and reproductive biology.
Product Name:
QuickStep Rat Estradiol ELISA Kit
Product Code:
QSES048
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Rat
Sensitivity:
1.17 pg/mL
Range:
3.13-200 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum, plasma, urine and saliva
Target:
Estradiol
Application:
This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum, plasma, urine, saliva.
Specificity:
This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Rat serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
10.77
26.39
77.92
11.54
28.98
71.99
Standard deviation
0.75
1.45
2.73
0.68
1.62
3.89
CV (%)
6.98
5.51
3.51
5.89
5.59
5.40
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
92-103
97
EDTA plasma(n=8)
86-99
91
Samples were spiked with high concentrations of Rat E2 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
