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Western Blot analysis of Human HEK293 cells overexpressing RAB3A, RAB3B, RAB3C, and RAB3D showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9 (STMA00219). Lane 1: MW ladder. Lanes 2-4: HEK293 cells overexpressing RAB3A. Lanes 5-7: HEK293 cells overexpressing RAB3B. Lanes 8-10: HEK293 cells overexpressing RAB3C. Lanes 11-13: HEK293 cells overexpressing RAB3D.
Key Features

Anti-RAB3D Antibody [5E9]

Datasheet
SKU STMA00219
Antibody Type Monoclonal
Applications WB
Reactivity Human
Reactivity Mouse
Reactivity Rat
Host Species Mouse
Isotype IgG1
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Description

RAB3D Antibody [5E9]

RAB3D Antibody [5E9] is a mouse monoclonal antibody directed against RAB3D, offered by Assay Genie for research applications. Reported applications include WB. Reported reactivity: Human, Mouse and Rat. Supplied as IgG1, purified by Protein G.

RAB3D is a member of the Rab family of small GTPases involved in regulated exocytosis. It plays a role in vesicle trafficking from the trans-Golgi network and is essential for protein secretion in various cell types. Although best known for its function in osteoclast-mediated bone resorption, RAB3D is also expressed in the nervous system, where it may influence synaptic vesicle dynamics and neurotransmitter release. Given the importance of vesicle trafficking in neuronal communication, dysregulation of RAB3D could contribute to synaptic dysfunction in neurodegenerative diseases. Its role in exocytosis makes it a candidate for further investigation in disorders characterized by impaired synaptic transmission. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.

Product Name:RAB3D Antibody [5E9]
Product SKU:STMA00219
Size:100 µg
Target:RAB3D
Host Species:Mouse
Antibody Type:Monoclonal
Clone ID:5E9
Research Areas:Cancer, Cell Signaling, Cell Signaling, Autophagy, Golgi Proteins, Alzheimer's Disease, Growth Factors, Membrane Trafficking Proteins, Neurodegeneration, Neuroscience, Parkinson's Disease, Protein Trafficking
Buffer:PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage & Shipping:Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:Liquid
Applications:WB
Antibody Isotype:IgG1
Purification:Protein G Purified
Concentration:1mg/ml
Reactivity:Human, Mouse, Rat
Recommended Dilution:WB (1:1000); optimal dilutions for assays should be determined by the user.
Specificity:Detects ~24 kDa. Does not show cross reactivity to RAB3A, RAB3B, or RAB3C.
Guarantee:12 months from date of dispatch
Immunogen:Full length recombinant Human RAB3D protein
Immunogen Species:Human
Accession Number:NP_004274
Gene ID:9545
Swiss-Prot:O95716
Cellular Localization:Cell membrane, Membrane
Alternative Names:Member RAS Oncogene Family, D2-2, GOV, RAB16, RAD3D, Ras-Related Protein Rab-3D
References:1. Zhu, S. et al. (2016) Sci Rep. 6:37963 2. Pavlos, N. J. et al. (2005) Mol Cell Biol. 25(12): 5253–5269
Note:For research use only.
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