Anti-RAB5C Antibody (CAB7342)

Antibody Name:	Anti-RAB5C Antibody
Antibody SKU:	CAB7342
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-216 of human RAB5C (NP_958842.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, BT-474, HL-60, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-216 of human RAB5C (NP_958842.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGR GGAA RPNG PAAG NKIC QFKL VLLG ESAV GKSS LVLR FVKG QFHE YQES TIGA AFLT QTVC LDDT TVKF EIWD TAGQ ERYH SLAP MYYR GAQA AIVV YDIT NTDT FARA KNWV KELQ RQAS PNIV IALA GNKA DLAS KRAV EFQE AQAY ADDN SLLF METS AKTA MNVN EIFM AIAK KLPK NEPQ NATG APGR NRGV DLQE NNPA SRSQ CCSN
Gene ID:	5878
Uniprot:	P51148
Cellular Location:	Cell membrane, Cytoplasmic side, Early endosome membrane, Lipid-anchor, Melanosome
Calculated MW:	23kDa/27kDa
Observed MW:	23kDa

Synonyms:	RAB5C, L1880, RAB5CL, RAB5L, RABL
Background:

UniProt Protein Function:	RAB5C: Protein transport. Probably involved in vesicular traffic. Belongs to the small GTPase superfamily. Rab family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; G protein, monomeric; G protein; G protein, monomeric, Rab Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: early endosome; endocytic vesicle; intracellular membrane-bound organelle; lipid particle; lysosomal membrane; plasma membrane Molecular Function:GDP binding; GTPase activity; protein binding Biological Process: plasma membrane to endosome transport; regulation of endocytosis
NCBI Summary:	Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).[supplied by OMIM, Nov 2010]
UniProt Code:	P51148
NCBI GenInfo Identifier:	38258923
NCBI Gene ID:	5878
NCBI Accession:	P51148.2
UniProt Secondary Accession:	P51148,Q6FH55, Q9P0Y5, F8W1H5,
UniProt Related Accession:	P51148
Molecular Weight:	27,036 Da
NCBI Full Name:	Ras-related protein Rab-5C
NCBI Synonym Full Names:	RAB5C, member RAS oncogene family
NCBI Official Symbol:	RAB5C
NCBI Official Synonym Symbols:	RABL; L1880; RAB5L; RAB5CL
NCBI Protein Information:	ras-related protein Rab-5C
UniProt Protein Name:	Ras-related protein Rab-5C
UniProt Synonym Protein Names:	L1880; RAB5L
Protein Family:	Ras-related protein
UniProt Gene Name:	RAB5C
UniProt Entry Name:	RAB5C_HUMAN

	Western blot analysis of extracts of various cell lines, using RAB5C antibody (CAB7342) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using RAB5C antibody (CAB7342) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney using RAB5C antibody (CAB7342) at dilution of 1:100 (40x lens).

Antibodies

RAB5C Antibody (PACO04861)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)