Anti-RAB7A Antibody (CAB12784)

Antibody Name:	Anti-RAB7A Antibody
Antibody SKU:	CAB12784
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human RAB7A (NP_004628.4).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, HeLa, Mouse brain, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human RAB7A (NP_004628.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DSWR DEFL IQAS PRDP ENFP FVVL GNKI DLEN RQVA TKRA QAWC YSKN NIPY FETS AKEA INVE QAFQ TIAR NALK QETE VELY NEFP EPIK LDKN DRAK ASAE SCSC
Gene ID:	7879
Uniprot:	P51149
Cellular Location:	Cytoplasmic side, Cytoplasmic vesicle, Late endosome membrane, Lipid droplet, Lysosome membrane, Melanosome membrane, Peripheral membrane protein, autophagosome membrane, phagosome membrane
Calculated MW:	23kDa
Observed MW:	23kDa

Synonyms:

RAB7A, PRO2706, RAB7

Background:

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

UniProt Protein Function:	Rab7: a member of the RAB family of RAS-related GTP-binding proteins, important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. Mutations in this protein cause Charcot-Marie-Tooth type 2B neuropathy.
UniProt Protein Details:	Protein type:G protein, monomeric; G protein, monomeric, Rab; G protein Chromosomal Location of Human Ortholog: 3q21.3 Cellular Component: Golgi apparatus; phagocytic vesicle membrane; intracellular membrane-bound organelle; lysosome; retromer complex; lysosomal membrane; late endosome; vacuolar membrane; pre-autophagosomal structure membrane; melanosome; phagocytic vesicle Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; Rac GTPase binding Biological Process: protein transport; protein targeting to lysosome; early endosome to late endosome transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; endosome to lysosome transport; endocytosis; protein to membrane docking; Rab protein signal transduction; retrograde transport, endosome to Golgi; epidermal growth factor ligand processing; bone resorption Disease: Charcot-marie-tooth Disease, Axonal, Type 2b
NCBI Summary:	RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]
UniProt Code:	P51149
NCBI GenInfo Identifier:	1709999
NCBI Gene ID:	7879
NCBI Accession:	P51149.1
UniProt Related Accession:	P51149
Molecular Weight:
NCBI Full Name:	Ras-related protein Rab-7a
NCBI Synonym Full Names:	RAB7A, member RAS oncogene family
NCBI Official Symbol:	RAB7A
NCBI Official Synonym Symbols:	RAB7; CMT2B; PRO2706
NCBI Protein Information:	ras-related protein Rab-7a
UniProt Protein Name:	Ras-related protein Rab-7a
Protein Family:	Ras-related protein
UniProt Gene Name:	RAB7A
UniProt Entry Name:	RAB7A_HUMAN

	Western blot analysis of extracts of various cell lines, using RAB7A antibody (CAB12784) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using RAB7A antibody (CAB12784) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using RAB7A antibody (CAB12784) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2OS cells using RAB7A antibody (CAB12784) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-RAB7A Antibody (CAB1154)

Anti-RAB7 Antibody (CAB12308)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)