The Rabbit CTGF (Connective Tissue Growth Factor) ELISA Kit is a highly reliable tool for quantitative detection of CTGF levels in rabbit serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for a variety of research applications. CTGF is a key regulatory protein involved in the growth and maintenance of connective tissue, playing a vital role in processes such as wound healing, fibrosis, and tissue remodeling. Dysregulation of CTGF expression has been implicated in various pathological conditions, including fibrotic diseases, cancer, and musculoskeletal disorders, making it a valuable target for research and therapeutic development.
By using the Rabbit CTGF ELISA Kit, researchers can gain insights into the role of CTGF in disease pathology and explore potential therapeutic strategies targeting this crucial growth factor. With its user-friendly format and excellent performance characteristics, this kit is an essential tool for studying CTGF biology and its implications in various health conditions.
Serum, Plasma, Cell Culture Supernatant, Cell Lysis Buffer or Tissue Lysis Buffer, and Other Biological Fluid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-104%
95-104%
86-102%
EDTA Plasma (n = 5)
82-101%
82-98%
96-100%
Heparin Plasma (n = 5)
84-99%
83-93%
81-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
90-100
94
EDTA Plasma (n = 5)
85-101
95
Heparin Plasma (n = 5)
85-99
92
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
