Rabbit Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
The Rabbit Macrophage Inflammatory Protein-1 Beta (MIP-1B) ELISA Kit is a highly reliable and sensitive tool for detecting MIP-1B levels in rabbit serum, plasma, and cell culture supernatants. With its high specificity and accuracy, this kit provides consistent and reproducible results, making it perfect for a variety of research applications.MIP-1B is a key protein involved in inflammatory responses and immune regulation.
Its role in conditions such as infection, autoimmune diseases, and inflammatory disorders makes it a valuable biomarker for studying these diseases and developing new treatment strategies.This ELISA kit offers researchers a powerful tool for studying the role of MIP-1B in various physiological and pathological processes, providing valuable insights into its mechanisms of action and potential therapeutic implications.
Product Name:
Rabbit Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Product Code:
RBDL00046
Size:
96 Assays
Target:
MIP1b
Synonyms:
CCL4, ACT2, G-26, LAG1, MIP1-B, SCYA4, Chemokine C-C-Motif Ligand 4, Small Inducible Cytokine A4, Homologous To Mouse Mip-1b
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
