The Rabbit Serum Amyloid A (SAA) ELISA Kit is specifically designed for the quantitative measurement of SAA levels in rabbit serum samples. This kit offers high sensitivity and specificity, allowing for accurate and reproducible results in various research applications.SAA is an acute-phase protein that plays a key role in inflammation and the immune response. Elevated levels of SAA have been associated with various inflammatory conditions, making it a valuable biomarker for studying these diseases and monitoring treatment effectiveness.
With the Rabbit Serum Amyloid A (SAA) ELISA Kit, researchers can easily assess SAA levels in rabbit samples, providing valuable insights into the inflammatory processes and potential therapeutic targets. This kit is an essential tool for studying inflammation-related disorders and developing novel treatment strategies.
Product Name:
Rabbit Serum Amyloid A (SAA) ELISA Kit
Product Code:
RBDL00067
Size:
96 Assays
Target:
SAA
Synonyms:
SA-A, SAA1, PIG4, TP53I4, Amyloid protein A, Amyloid fibril protein AA, Serum amyloid A-1 protein
Detection Method:
Sandwich
Range:
0.156-10?g/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
