Description

Rabbit Sod2 (Superoxide dismutase [Mn], mitochondrial) ELISA Kit

The Rabbit SOD2 (Superoxide Dismutase Mn) Mitochondrial ELISA Kit is specifically designed for the precise measurement of SOD2 levels in rabbit samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes. SOD2 is an essential enzyme that plays a critical role in antioxidant defense by catalyzing the dismutation of superoxide radicals in the mitochondria.

Imbalances in SOD2 levels have been linked to oxidative stress-related diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions. By accurately measuring SOD2 levels, researchers can gain valuable insights into the pathogenesis of these diseases and explore potential therapeutic interventions.

Linearity:

Sample	1:2	1:4	1:8
Serum (n = 5)	85-88%	92-98%	85-104%
EDTA Plasma (n = 5)	89-100%	89-99%	82-101%
Heparin Plasma (n = 5)	83-97%	81-99%	80-97%

Recovery:

Sample	Recovery Range (%)	Average (%)
Serum (n = 5)	98-103	93
EDTA Plasma (n = 5)	91-101	95
Heparin Plasma (n = 5)	89-103	93

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Product Name:	Rabbit Sod2 (Superoxide dismutase [Mn], mitochondrial) ELISA Kit
SKU:	RBFI00150
Reactivity:	Rabbit
Assay Type:	Sandwich ELISA, Double Antibody
Sensitivity:	0.094 ng/mL
Range:	0.156-10 ng/mL
Sample Type:	Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:	2-8°C for 12 months.

Step	Procedure
1	Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2	Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3	Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4	HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5	Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6	Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.

Sample Type	Protocol
Serum	Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma	Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate	Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant	Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate	Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types	For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.

Component	Quantity		Storage
	48T	96T
ELISA Microplate (Dismountable)	8×6	8×12	Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard	1 vial	2 vial	Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Concentrated, 100X)	60 ul	120 ul	2-8°C (Avoid direct light)
HRP-Streptavidin Conjugate (SABC, 100X)	60 ul	120 ul	2-8°C (Avoid direct light)
TMB Substrate	5 ml	10 ml	2-8°C (Avoid direct light)
Sample Dilution Buffer	10 ml	20 ml	2-8°C
Antibody Dilution Buffer	5 ml	10 ml	2-8°C
SABC Dilution Buffer	5 ml	10 ml	2-8°C
Stop Solution	5 ml	10 ml	2-8°C
Wash Buffer(25X)	15 ml	30 ml	2-8°C
Plate Sealer	3 pieces	5 pieces	-
Technical Manual	1 copy	1 copy	-

Rabbit Sod2 (Superoxide dismutase [Mn], mitochondrial) ELISA Kit (RBFI00150)

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