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Epigenetics and Nuclear Signaling Antibodies 5 Anti-Rad51 Antibody CAB2829
Key Features

Rad51 Rabbit Monoclonal Antibody (CAB2829)

Datasheet MSDS
SKU CAB2829
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms RECA, BRCC5, FANCR, MRMV2, HRAD51, RAD51A, HsRad51, HsT16930, Rad51
Research Use Epigenetics and Nuclear Signaling
$173 - $603

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Description

system_update_altDatasheetsystem_update_altMSDS

Rad51 Monoclonal Antibody (CAB2829)

The Rad51 Monoclonal Antibody (CAB2829) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically tailored for use in immunofluorescence and Western blot techniques, providing accurate and reliable detection of RAD51 protein levels in various sample types.RAD51 is a crucial protein involved in the repair of DNA double-strand breaks, playing a vital role in maintaining genomic stability and preventing the accumulation of mutations. Dysregulation of RAD51 function has been linked to various diseases, including cancer, highlighting its importance in maintaining cellular integrity.

This antibody is validated for use in WB, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Rad51 Monoclonal Antibody
SKU:CAB2829
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC0764
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MAMQ MQLE ANAD TSVE EESF GPQP ISRL EQCG INAN DVKK LEEA GFHT VEAV AYAP KKEL INIK GISE AKAD KILA EAAK LVPM GFTT ATEF HQRR SEII
Tested Applications:WB ChIP ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP5μg antibody for 10μg-15μg of Chromatin
Synonyms:RECA, BRCC5, FANCR, MRMV2, HRAD51, RAD51A, HsRad51, HsT16930, Rad51
Positive Sample:HeLa, Jurkat, NIH/3T3, C6
Cellular Localization:Cytoplasm, Mitochondrion Matrix, Nucleus, Centrosome, Cytoskeleton, Microtubule Organizing Center, Perinuclear Region.
Calculated MW:37kDa
Observed MW:37kDa

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID5888
RRIDAB_2863021
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Rad51 Rabbit mAb (CAB2829) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Product ImageChromatin immunoprecipitation analysis of extracts of HepG2 cells, using Rad51 Rabbit mAb antibody (CAB2829) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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