Anti-RAMP1 Antibody (CAB6447)

Antibody Name:	Anti-RAMP1 Antibody
Antibody SKU:	CAB6447
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 27-117 of human RAMP1 (NP_005846.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 27-117 of human RAMP1 (NP_005846.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CQEA NYGA LLRE LCLT QFQV DMEA VGET LWCD WGRT IRSY RELA DCTW HMAE KLGC FWPN AEVD RFFL AVHG RYFR SCPI SGRA VRDP PGS
Gene ID:	10267
Uniprot:	O60894
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	16kDa
Observed MW:	17kDa

Synonyms:

RAMP1

Background:

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	RAMP1: Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL. Belongs to the RAMP family.
UniProt Protein Details:	Protein type:Receptor, GPCR; Membrane protein, integral Chromosomal Location of Human Ortholog: 2q36-q37.1 Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane; receptor complex Molecular Function:protein binding; calcitonin receptor binding; coreceptor activity; receptor activity; protein transporter activity; calcitonin receptor activity Biological Process: intracellular protein transport; G-protein coupled receptor protein signaling pathway; protein transport; positive regulation of protein amino acid glycosylation; receptor internalization; calcium ion transport; positive regulation of cAMP biosynthetic process; angiogenesis; regulation of G-protein coupled receptor protein signaling pathway
NCBI Summary:	The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface. [provided by RefSeq, Jul 2008]
UniProt Code:	O60894
NCBI GenInfo Identifier:	11134419
NCBI Gene ID:	10267
NCBI Accession:	O60894.1
UniProt Secondary Accession:	O60894,Q6FGS5,
UniProt Related Accession:	O60894
Molecular Weight:	148
NCBI Full Name:	Receptor activity-modifying protein 1
NCBI Synonym Full Names:	receptor (G protein-coupled) activity modifying protein 1
NCBI Official Symbol:	RAMP1
NCBI Protein Information:	receptor activity-modifying protein 1; CRLR activity-modifying protein 1; receptor activity modifying protein 1; receptor (calcitonin) activity modifying protein 1; calcitonin receptor-like receptor activity modifying protein 1; calcitonin-receptor-like receptor activity-modifying protein 1
UniProt Protein Name:	Receptor activity-modifying protein 1
UniProt Synonym Protein Names:	Calcitonin-receptor-like receptor activity-modifying protein 1; CRLR activity-modifying protein 1
Protein Family:	Receptor activity-modifying protein
UniProt Gene Name:	RAMP1
UniProt Entry Name:	RAMP1_HUMAN

	Western blot analysis of extracts of HepG2 cells, using RAMP1 antibody (CAB6447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of NIH/3T3 cells using RAMP1 antibody (CAB6447) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using RAMP1 antibody (CAB6447) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
RAMP1 Antibody (PACO11704)	Human RAMP1 (Receptor activity-modifying protein 1) ELISA Kit (HUFI08029)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)