Anti-RAN Antibody (CAB0976)

Antibody Name:	Anti-RAN Antibody
Antibody SKU:	CAB0976
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat, Monkey
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-216 of human RAN (NP_006316.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat, Monkey
Positive Samples:	NIH/3T3, COS-1, COS-7, Jurkat, 293T, Mouse testis, rat testis, C6, mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-216 of human RAN (NP_006316.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAQ GEPQ VQFK LVLV GDGG TGKT TFVK RHLT GEFE KKYV ATLG VEVH PLVF HTNR GPIK FNVW DTAG QEKF GGLR DGYY IQAQ CAII MFDV TSRV TYKN VPNW HRDL VRVC ENIP IVLC GNKV DIKD RKVK AKSI VFHR KKNL QYYD ISAK SNYN FEKP FLWL ARKL IGDP NLEF VAMP ALAP PEVV MDPA LAAQ YEHD LEVA QTTA LPDE DDDL
Gene ID:	5901
Uniprot:	P62826
Cellular Location:	Cytoplasm, Melanosome, Nucleus, Nucleus envelope
Calculated MW:	24kDa
Observed MW:	23kDa

Synonyms:

RAN, ARA24, Gsp1, TC4

Background:

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

UniProt Protein Function:	RAN: a small GTPase belonging to the RAS superfamily that regulates the nucleo-cytoplasmic traffic through the nuclear pore complex, the condensation of chromatin, spindle assembly and cytokinesis, as well as the organization of non-centrosomal microtubules. A major regulator of mitosis and a potential therapeutic target in cancers expressing higher Ras/MEK/ERK and PI3K/Akt/mTORC1 activities. Required for the import of protein into and RNA export from the nucleus. Cycles between the nucleus and cytoplasm in this process. The Ran guanine nucleotide-exchange factor (RanGEF) is nuclear, promoting the GTP-bound state, while the Ran GTPase activating protein (RanGAP) is cytoplasmic, promoting GDP-bound state. RanGDP is imported into the nucleus by nuclear transport factor-2 (NUTF2), where RanGEF catalyses nucleotide exchange and generates RanGTP. RanGTP then binds to transport factors, such as KPNB1 and CAS, and is exported to the cytoplasm, where RanGAP stimulates GTP hydrolysis. Regulates microtubule polymerization during mitosis; micritubule localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1), a Ran-GEF. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Regulates the formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. GTP-Ran interacts with survivin at S and M phases of the cell cycle. Ran-survivin complexes play a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Interacts with TERT following the tyrosine phosphorylation of TERT induced by H2O2, inducing nuclear export of TERT. Interacts with MAD2L2. Predominantly nuclear during interphase. Becomes dispersed throughout the cytoplasm during mitosis.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cell cycle regulation; Nuclear receptor co-regulator; G protein, monomeric, Ran; G protein, monomeric; Nuclear export; G protein Chromosomal Location of Human Ortholog: 12q24.3 Cellular Component: centriole; chromatin; cytoplasm; cytosol; membrane; midbody; nucleolus; nucleoplasm; nucleus; recycling endosome Molecular Function:chromatin binding; GDP binding; GTP binding; GTPase activity; protein binding Biological Process: DNA metabolic process; mitosis; mitotic spindle organization and biogenesis; positive regulation of protein binding; pre-microRNA export from nucleus; protein export from nucleus; protein import into nucleus, translocation; ribosomal large subunit export from nucleus; ribosomal small subunit export from nucleus; RNA-mediated gene silencing; signal transduction; tRNA export from nucleus; viral reproduction
NCBI Summary:	RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq, Jul 2008]
UniProt Code:	P62826
NCBI GenInfo Identifier:	51338598
NCBI Gene ID:	5901
NCBI Accession:	P62826.3
UniProt Secondary Accession:	P62826,P17080, P28746, P28747, Q6IPB2, Q86V08, Q8NI90 Q9CSP3, Q9CWI7, Q9CZA2, Q9UDJ5, A8K3Z8,
UniProt Related Accession:	P62826,AAB24940
Molecular Weight:	24,423 Da
NCBI Full Name:	GTP-binding nuclear protein Ran
NCBI Synonym Full Names:	RAN, member RAS oncogene family
NCBI Official Symbol:	RAN
NCBI Official Synonym Symbols:	TC4; Gsp1; ARA24
NCBI Protein Information:	GTP-binding nuclear protein Ran
UniProt Protein Name:	GTP-binding nuclear protein Ran
UniProt Synonym Protein Names:	Androgen receptor-associated protein 24; GTPase Ran; Ras-like protein TC4; Ras-related nuclear protein
Protein Family:	Ranakinin
UniProt Gene Name:	RAN
UniProt Entry Name:	RAN_HUMAN

Western blot analysis of extracts of various cell lines, using RAN antibody (CAB0976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Antibodies

Anti-Ran Antibody (CAB4374)

RAN Antibody (PACO01406)

RAN Antibody (PACO11707)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)