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Anti-Ran Antibody (CAB4374)

SKU:
CAB4374
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
ARA24
Gsp1
TC4
  • Cell Cycle Antibodies 2 Anti-Ran Antibody CAB4374
  • Cell Cycle Antibodies 2 Anti-Ran Antibody CAB4374
€139 - €419
Frequently bought together:

Description

Product Name:Ran Rabbit mAb
Product Code:CAB4374
Size:20uL, 50uL, 100uL
Synonyms:ARA24, Gsp1, TC4
Applications:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Ran
Applications:WB
Recommended Dilutions:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, Mouse brain, Mouse spleen, Mouse thymus, Rat testis
Immunogen:A synthesized peptide derived from human Ran
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5901
Uniprot:P62826
Cellular Location:Cytoplasm, Melanosome, Nucleus, Nucleus envelope
Calculated MW:24kDa
Observed MW:24KDa
UniProt Protein Function:RAN: a small GTPase belonging to the RAS superfamily that regulates the nucleo-cytoplasmic traffic through the nuclear pore complex, the condensation of chromatin, spindle assembly and cytokinesis, as well as the organization of non-centrosomal microtubules. A major regulator of mitosis and a potential therapeutic target in cancers expressing higher Ras/MEK/ERK and PI3K/Akt/mTORC1 activities. Required for the import of protein into and RNA export from the nucleus. Cycles between the nucleus and cytoplasm in this process. The Ran guanine nucleotide-exchange factor (RanGEF) is nuclear, promoting the GTP-bound state, while the Ran GTPase activating protein (RanGAP) is cytoplasmic, promoting GDP-bound state. RanGDP is imported into the nucleus by nuclear transport factor-2 (NUTF2), where RanGEF catalyses nucleotide exchange and generates RanGTP. RanGTP then binds to transport factors, such as KPNB1 and CAS, and is exported to the cytoplasm, where RanGAP stimulates GTP hydrolysis. Regulates microtubule polymerization during mitosis; micritubule localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1), a Ran-GEF. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Regulates the formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. GTP-Ran interacts with survivin at S and M phases of the cell cycle. Ran-survivin complexes play a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Interacts with TERT following the tyrosine phosphorylation of TERT induced by H2O2, inducing nuclear export of TERT. Interacts with MAD2L2. Predominantly nuclear during interphase. Becomes dispersed throughout the cytoplasm during mitosis.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cell cycle regulation; Nuclear receptor co-regulator; G protein, monomeric, Ran; G protein, monomeric; Nuclear export; G protein

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: centriole; chromatin; cytoplasm; cytosol; membrane; midbody; nucleolus; nucleoplasm; nucleus; recycling endosome

Molecular Function:chromatin binding; GDP binding; GTP binding; GTPase activity; protein binding

Biological Process: DNA metabolic process; mitosis; mitotic spindle organization and biogenesis; positive regulation of protein binding; pre-microRNA export from nucleus; protein export from nucleus; protein import into nucleus, translocation; ribosomal large subunit export from nucleus; ribosomal small subunit export from nucleus; RNA-mediated gene silencing; signal transduction; tRNA export from nucleus; viral reproduction

NCBI Summary:RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq, Jul 2008]
UniProt Code:P62826
NCBI GenInfo Identifier:51338598
NCBI Gene ID:5901
NCBI Accession:P62826.3
UniProt Secondary Accession:P62826,P17080, P28746, P28747, Q6IPB2, Q86V08, Q8NI90 Q9CSP3, Q9CWI7, Q9CZA2, Q9UDJ5, A8K3Z8,
UniProt Related Accession:P62826,AAB24940
Molecular Weight:24,423 Da
NCBI Full Name:GTP-binding nuclear protein Ran
NCBI Synonym Full Names:RAN, member RAS oncogene family
NCBI Official Symbol:RAN  
NCBI Official Synonym Symbols:TC4; Gsp1; ARA24  
NCBI Protein Information:GTP-binding nuclear protein Ran
UniProt Protein Name:GTP-binding nuclear protein Ran
UniProt Synonym Protein Names:Androgen receptor-associated protein 24; GTPase Ran; Ras-like protein TC4; Ras-related nuclear protein
Protein Family:Ranakinin
UniProt Gene Name:RAN  
UniProt Entry Name:RAN_HUMAN

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