Anti-RASGRP1 Antibody (CAB10495)

Antibody Name:	Anti-RASGRP1 Antibody
Antibody SKU:	CAB10495
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 598-797 of human RASGRP1 (NP_005730.2).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human
Positive Samples:	Jurkat, MCF7, A-549, U-251MG, SW480

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 598-797 of human RASGRP1 (NP_005730.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PVAP TENN TSVG PVSN LCSL GAKD LLHA PEEG PFTF PNGE AVEH GEES KDRT IMLM GVSS QKIS LRLK RAVA HKAT QTES QPWI GSEG PSGP FVLS SPRK TAQD TLYV LPSP TSPC PSPV LVRK RAFV KWEN KDSL IKSK EELR HLRL PTYQ ELEQ EINT LKAD NDAL KIQL KYAQ KKIE SLQL EKSN HVLA QMEQ GDCS
Gene ID:	10125
Uniprot:	O95267
Cellular Location:	Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Golgi apparatus membrane, Peripheral membrane protein, cytosol
Calculated MW:	55kDa/62kDa/68kDa/86kDa/90kDa
Observed MW:	90kDa

Synonyms:

RASGRP1, CALDAG-GEFI, CALDAG-GEFII, RASGRP, V, hRasGRP1

Background:

This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE).

UniProt Protein Function:	RASGRP1: Functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Couples T-lymphocytes and B-lymphocytes antigen receptors to the activation of Ras. Hence, regulates T-cells and B-cells development, homeostasis and differentiation. Functions also in FcERI-evoked degranulation and cytokine secretion by mast cells, regulating allergic responses. May also function in other cell types differentiation. Defects in RASGRP1 may contribute to susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Aberrantly spliced isoforms and/or diminished levels of RASGRP1 are found in a cohort of SLE patients raising the possibility that dysregulation of this signaling protein contributes to the development of autoimmunity in a subset of SLE patients. Belongs to the RASGRP family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GEF; GEF, Ras Chromosomal Location of Human Ortholog: 15q14 Cellular Component: cytosol; Golgi apparatus; membrane; plasma membrane Molecular Function:calcium ion binding; diacylglycerol binding; guanyl-nucleotide exchange factor activity; lipid binding; phosphatidylcholine binding; phosphatidylserine binding; protein homodimerization activity; Ras guanyl-nucleotide exchange factor activity; zinc ion binding Biological Process: MAPKKK cascade; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of GTPase activity; positive regulation of JNK cascade; positive regulation of MAP kinase activity; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of protein amino acid phosphorylation; positive regulation of Ras protein signal transduction; positive regulation of tumor necrosis factor production; protein oligomerization; signal transduction
NCBI Summary:	This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE). [provided by RefSeq, Jul 2008]
UniProt Code:	O95267
NCBI GenInfo Identifier:	166987610
NCBI Gene ID:	10125
NCBI Accession:	O95267.2
UniProt Secondary Accession:	O95267,Q56CZ0, Q58G75, Q59HB1, Q5I3A8, Q6GV31, Q6NX39 Q7LDG6, Q9UI94, Q9UNN9,
UniProt Related Accession:	O95267
Molecular Weight:	90kDa
NCBI Full Name:	RAS guanyl-releasing protein 1
NCBI Synonym Full Names:	RAS guanyl releasing protein 1
NCBI Official Symbol:	RASGRP1
NCBI Official Synonym Symbols:	V; RASGRP; hRasGRP1; CALDAG-GEFI; CALDAG-GEFII
NCBI Protein Information:	RAS guanyl-releasing protein 1
UniProt Protein Name:	RAS guanyl-releasing protein 1
UniProt Synonym Protein Names:	Calcium and DAG-regulated guanine nucleotide exchange factor II; CalDAG-GEFII; Ras guanyl-releasing protein
Protein Family:	RAS guanyl-releasing protein
UniProt Gene Name:	RASGRP1

Western blot analysis of extracts of various cell lines, using RASGRP1 antibody (CAB10495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)