Rat Adenylate Cyclase 10, Soluble (ADCY10) ELISA Kit
The Rat Adenylate Cyclase 10 Soluble (ADCY10) ELISA Kit is a powerful tool for precise measurement of ADCY10 levels in rat tissue lysates, serum, and plasma samples. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research studies.ADCY10 is a key enzyme involved in cAMP signaling pathways, playing a critical role in regulating various cellular processes such as cell growth, differentiation, and metabolism. Dysregulation of ADCY10 has been implicated in a variety of diseases including cancer, diabetes, and cardiovascular disorders, highlighting its significance as a potential therapeutic target and diagnostic marker.
With the Rat Adenylate Cyclase 10 Soluble ELISA Kit, researchers can explore the role of ADCY10 in different physiological and pathological conditions, paving the way for advancements in understanding and treating various disorders. Get reliable data and valuable insights with this user-friendly and efficient ELISA kit from AssayGenie.
Product Name:
Rat Adenylate Cyclase 10, Soluble (ADCY10) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
