Rat Amyloid Beta Precursor Protein Binding Protein 2 (APPBP2) ELISA Kit
The Rat APPBP2 (Amyloid Beta Precursor Protein Binding Protein 2) ELISA Kit is specifically designed for the accurate quantification of APPBP2 levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.APPBP2 is a key protein that binds to the amyloid precursor protein (APP) and plays a role in regulating the processing of amyloid beta, a peptide implicated in Alzheimer's disease.
Abnormal APPBP2 levels have been linked to neurodegenerative disorders, making this ELISA kit a valuable tool for investigating these conditions and developing potential therapeutic strategies.Overall, the Rat APPBP2 ELISA Kit provides researchers with a powerful tool for studying the role of APPBP2 in neurodegenerative diseases and other related conditions, making it an essential component of any research program focused on understanding the molecular mechanisms underlying these disorders.
Product Name:
Rat Amyloid Beta Precursor Protein Binding Protein 2 (APPBP2) ELISA Kit
Product Code:
RTDL00103
Size:
96 Assays
Target:
APPBP2
Synonyms:
PAT1, Protein Interacting With APP Tail 1,Cytoplasmic Tail, Amyloid protein-binding protein 2, Protein interacting with APP tail 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
