The Rat apoA1 (Apolipoprotein A1) CLIA Kit is specifically designed for the quantitative detection of Apolipoprotein A1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Apolipoprotein A1 is an important component of high-density lipoprotein (HDL) and plays a key role in lipid metabolism and cardiovascular health. Abnormal levels of Apolipoprotein A1 have been associated with various cardiovascular diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Rat apoA1 (Apolipoprotein A1) CLIA Kit is a reliable tool for researchers looking to investigate the role of Apolipoprotein A1 in physiological and pathological processes related to cardiovascular health in rat models.
Product Name:
Rat ApoA1 (Apolipoprotein A1) CLIA Kit
SKU:
RTES00610
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
