The Rat Bcl-2 (B-cell Lymphoma/Leukemia 2) ELISA Kit is a specialized assay meticulously designed to quantitatively measure and analyze the levels of Bcl-2 in various biological samples from rats. Bcl-2, a critical anti-apoptotic protein, is known for its pivotal role in regulating programmed cell death and maintaining cellular homeostasis. Dysregulation in Bcl-2 expression has been linked to numerous diseases, including cancer and autoimmune disorders, emphasizing the significance of precise quantification of Bcl-2 levels in research studies. This ELISA kit boasts exceptional sensitivity and specificity, ensuring the generation of accurate and reproducible data, thereby enabling researchers to delve deeper into the intricate molecular mechanisms modulated by Bcl-2. Manufactured under stringent quality control standards, the Rat Bcl-2 ELISA Kit guarantees robust performance and user-friendly operation, making it an optimal choice for researchers investigating the functions and therapeutic potential of Bcl-2 in diverse biological processes and disease pathologies. Trust in this kit to drive your research forward and unlock valuable insights into the role of Bcl-2 in cellular health and disease.
Product Name:
Rat Bcl-2 (B-cell Lymphoma/Leukemia 2) ELISA Kit
SKU:
AEES00322
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
