The Rat Biliverdin Reductase A (BLVRA) ELISA Kit is a valuable tool for the precise measurement of BLVRA levels in rat samples including serum, plasma, and cell culture supernatants. With superior sensitivity and specificity, this kit delivers accurate and reproducible results, making it suitable for various research applications.BLVRA is an important enzyme involved in the conversion of biliverdin to bilirubin, a critical step in the metabolism of heme. Dysregulation of BLVRA has been linked to various diseases including liver disorders, cardiovascular diseases, and oxidative stress-related conditions.
Therefore, monitoring BLVRA levels can offer valuable insights into these pathologies and aid in the development of potential therapeutic interventions. Get reliable and consistent data with the Rat Biliverdin Reductase A (BLVRA) ELISA Kit, and advance your research in the field of heme metabolism and associated diseases.
Product Name:
Rat Biliverdin Reductase A (BLVRA) ELISA Kit
Product Code:
RTDL00143
Size:
96 Assays
Target:
BLVRA
Synonyms:
BLVR, BVR, Biliverdin-IX alpha-reductase
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
