Rat Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) ELISA Kit
The Rat Calcium/Calmodulin-dependent Protein Kinase II Alpha (CAMK2A) ELISA Kit is a powerful tool for accurately measuring levels of CAMK2A in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it perfect for a wide range of research applications.CAMK2A is a key enzyme involved in various cellular processes, playing a critical role in memory formation, synaptic plasticity, and neuronal development.
Dysregulation of CAMK2A has been linked to neurological disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.Get precise and dependable results with the Rat CAMK2A ELISA Kit, allowing you to advance your research and gain valuable insights into the role of CAMK2A in health and disease.
Product Name:
Rat Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) ELISA Kit
Product Code:
RTDL00169
Size:
96 Assays
Target:
CAMK2a
Synonyms:
CAMKA
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
