Rat Cannabinoid Receptor 1, Brain (CNR1) ELISA Kit
The Rat Cannabinoid Receptor 1 (Brain CNR1) ELISA Kit is specifically designed for the precise measurement of CNR1 levels in rat brain tissue extracts. This kit provides exceptional sensitivity and accuracy, leading to dependable and consistent results, making it an excellent choice for various research purposes.CNR1, also known as the cannabinoid receptor 1, plays a crucial role in the endocannabinoid system, influencing a variety of physiological processes such as pain sensation, appetite regulation, and mood.
Dysregulation of the CNR1 receptor has been linked to numerous health conditions, including psychiatric disorders, chronic pain, and addiction. Therefore, studying CNR1 levels can provide valuable insights into these conditions and aid in the development of potential therapies.With the Rat Cannabinoid Receptor 1 (Brain CNR1) ELISA Kit, researchers can accurately quantify CNR1 levels in rat brain tissue samples, facilitating in-depth investigations and discoveries in the field of cannabinoid research.
Product Name:
Rat Cannabinoid Receptor 1, Brain (CNR1) ELISA Kit
Product Code:
RTDL00223
Size:
96 Assays
Target:
CNR1
Synonyms:
CANN6, CB-R, CB1, CB1A, CB1K5, CNR
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
