Rat Carboxypeptidase A2, Pancreatic (CPA2) ELISA Kit
The Rat Carboxypeptidase A2 (Pancreatic CPA2) ELISA Kit is a reliable and easy-to-use kit designed for the accurate detection of carboxypeptidase A2 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Carboxypeptidase A2 is an important enzyme involved in the breakdown of peptides and proteins, playing a critical role in various biological processes.
Dysregulation of carboxypeptidase A2 has been implicated in diseases such as pancreatic cancer, inflammatory disorders, and metabolic diseases, highlighting its significance as a potential therapeutic target and biomarker.With the Rat Carboxypeptidase A2 (Pancreatic CPA2) ELISA Kit, researchers can accurately measure carboxypeptidase A2 levels in rat samples, providing valuable insights into disease mechanisms and potentially leading to the development of novel treatment strategies.
Product Name:
Rat Carboxypeptidase A2, Pancreatic (CPA2) ELISA Kit
Product Code:
RTDL00240
Size:
96 Assays
Target:
CPA2
Detection Method:
Sandwich
Range:
1.56 -100 U/L
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
