The Rat Caspase-14 (CASP14) ELISA Kit is specifically designed for the accurate detection of caspase-14 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.Caspase-14 is a key protein involved in apoptosis, the programmed cell death process essential for maintaining tissue homeostasis and eliminating damaged cells. Dysregulation of caspase-14 has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions, highlighting its importance as a biomarker for studying and potentially treating these ailments.
With its reliable performance and user-friendly protocol, the Rat Caspase-14 ELISA Kit is an invaluable tool for researchers looking to delve deeper into the role of caspase-14 in disease pathology and therapeutic interventions. Visit the provided link for more information and to order your kit today.
Product Name:
Rat Caspase 14 (CASP14) ELISA Kit
Product Code:
RTDL00174
Size:
96 Assays
Target:
CASP14
Synonyms:
Caspase 14 Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 14
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
