The Rat CHGA (Chromogranin A) CLIA Kit is a cutting-edge assay designed for the precise measurement of chromogranin A levels in rat biological samples. This kit offers outstanding sensitivity and specificity, guaranteeing accurate and consistent results, making it a valuable tool for various research purposes.Chromogranin A is a key protein involved in neuroendocrine functions, serving as a marker for neuroendocrine tumors and stress responses.
Its levels are also linked to conditions like hypertension, heart failure, and neurological disorders, highlighting its importance as a biomarker in these areas of study.Overall, the Rat CHGA CLIA Kit from Assay Genie provides researchers with a reliable and efficient solution for investigating the role of chromogranin A in health and disease, paving the way for advancements in diagnosis and treatment strategies.
Product Name:
Rat CHGA (Chromogranin A) CLIA Kit
SKU:
RTES00119
Target:
Rat CHGA (Chromogranin A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CHGA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CHGA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CHGA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CHGA. You can calculate the concentration of Rat CHGA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
103-119
98-113
Average (%)
93
109
104
1:4
Range (%)
100-113
86-99
87-102
Average (%)
106
91
93
1:8
Range (%)
84-100
99-114
101-116
Average (%)
91
106
107
1:16
Range (%)
93-104
86-100
91-105
Average (%)
98
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
83-97
90
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.18
80.06
371.05
39.09
84.93
347.88
Standard deviation
4.05
9.1
23.08
3.21
8.77
21.99
C V (%)
9.6
11.37
6.22
8.21
10.33
6.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CHGA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CHGA in samples. No significant cross-reactivity or interference between Rat CHGA and analogues was observed.
