The Rat Collagen Type III Alpha 1 (COL3A1) ELISA Kit is specifically designed for the precise measurement of COL3A1 levels in rat samples including serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for a variety of research studies.Collagen Type III Alpha 1 is a vital component of the extracellular matrix, playing a key role in tissue regeneration, wound healing, and structural support.
Abnormal levels of COL3A1 have been linked to various diseases and conditions such as fibrosis, cardiovascular diseases, and connective tissue disorders. Therefore, this ELISA kit provides researchers with essential information for studying these conditions and potentially identifying new therapeutic targets.
Product Name:
Rat Collagen Type III Alpha 1 (COL3a1) ELISA Kit
Product Code:
RTDL00231
Size:
96 Assays
Target:
COL3a1
Synonyms:
COL3-A1, COL3A1, EDS4A, Collagen Type III Alpha 1, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, Collagen Alpha-1(III)chain
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
